Mammalian Antibody Light Chai麗些n Gene Expression Vector

Recombinant antibodies have ext數妹ensive applications in 間筆the fields of d鄉好iagnostics and therapeutic medicine. Cu們醫rrently, there are about 180 mon近城oclonal antibody products appr又市oved worldwide targeting specifi從們c proteins, such白習 as PD-L1, HER2不數, IL-17R, and VEGF. The dev就睡elopment of genet為訊ic engineering has facili低草tated successful production of 費玩these therapeutic ant短喝ibodies, which have ideal tumor pen問業etration behavior,日來 short retention times, and reduced im明你munogenicity. These 男廠therapeutic recombinant antibodies冷視 are mainly produced i他畫n mammalian cells in vitro (e.門離g., CHO cells), since the電文 post-transcriptional modification in t通購his expression system is closest to the兵跳 in vivo processe器月s in humans. 

VectorBuilder’s mamma很月lian antibody light chain gene expres林很sion vector has been designed音東 specifically for high-yield producti購相on of monoclonal antibody light跳空 chains. All antibodies明輛 contain heavy and light chain司雪s, both of which consist上地 of variable (V) an議多d constant (C) regions. In mammals, an媽信tibodies are divi這電ded into five i睡看sotypes (IgG, IgA, I熱煙gM, IgD and IgE) based on the heavy c草拍hain C region sequence. A giv報南en mammalian antibody isotype eith看呢er has lambda (λ) or kap空歌pa (κ) light chains which ar分購e encoded by the light chain C店術 region. While there紅區 is no apparent functional difference科了, the ratio of the two types of 購花light chains varies fro街筆m species to species. The light chain p業遠lays a role in preventing花事 self-reactivity, and there is g多人rowing evidence that it 日明enhances receptor activ裡吃ation through homotypic 樂我interactions.

In addition to this cons農聽tant region, th機黑e V regions in 術電heavy and light chains contribute to相購 antigen recognition. The兵明refore, by customizing s你會equences encoding 遠熱the light chain V region, customers m鐘窗ay generate vectors to satisfy thei說村r personalized need for anti紙金gen-binding specificit新你ies. In the vector de為秒sign process, the light chain C 議森region may either be selected f畫子rom our popular light chain C region廠相 database (containing human κ 報嗎and λ2 light chains, and mouse κ, λ1謝你, λ2 light chains) or be pasted 年訊by customers using their own seq木請uence. Additionally, si鄉生gnal peptides (e.g., IL-2 sig) can 你體be added into the ve冷資ctor to increase secre些畫tion of the recombinant antibody.

For further information about th樹體is vector system, please r少森efer to the pape在湖rs below.