Mammalian Antibody Heavy and 化哥Light Chain Coexpressi討劇on Vector

Recombinant antibodies have e街場xtensive applications in the field近黑s of diagnostics and t吃件herapeutic medicine. Currently, t電鐵here are about 180 monoclonal antibod西是y products approved worl銀微dwide targeting specific protei務房ns, such as PD-L1, HER2, IL-17R, 他地and VEGF. The development of gene時雪tic engineering has黃服 facilitated success要他ful production of these therapeu也化tic antibodies, which ha間到ve ideal tumor penetrati為作on behavior, short水快 retention times, an慢時d reduced immunogenicity熱信. These therape廠到utic recombinant antibo飛視dies are mainly 志聽produced in mammalian cells i用務n vitro (e.g., CHO cells), since the那湖 post-transcriptional modification in t相雜his expression system is close白朋st to the in vivo proces討北ses in humans.

VectorBuilder’s mammalian anti報土body heavy and light chain c兒服oexpression vector has been designe聽看d specifically for 笑人high-yield production o數市f monoclonal antibody h就空eavy and light chains. All antibodi要公es contain heavy and 多得light chains, both o數通f which consist of variable (V) a器黑nd constant (C) regions. The an姐村tigen-binding site consists 些生of VH+VL dimers where VH and VL re木女present variable fragment heavy ch通場ains and light chains她妹, respectively. Within these 懂海fragments, most seque街費nce variation is located in comple拿兒mentarity deter生要mining regions (CDRs), w算拿hich influences antigen-bin有動ding specificity. In mammals, an美媽tibodies are divided into f兒業ive isotypes (IgG, IgA, IgM, IgD and Ig美議E) based on the he中電avy chain C region sequence. Of the作美 five immunoglobulin (an街內tibody) isotypes, IgG is the女公 most abundant in circulation. Moreov但煙er, almost all ba南放ckbones of the appro如笑ved therapeutic antibodies are IgG. An我行 antibody of a gi事公ven isotype either has lambda (λ) or ka站鐘ppa (κ) light chains, which ar聽音e encoded by the light chain C r快你egion. While the heavy chain is typica線市lly associated with target spe鐘姐cificity, the l爸金ight chain is as筆開sociated with preventing森術 self-reactivity and en光議hancing receptor activation. The ratio校個 of the two types of light chain紅街s varies from species to species.什冷

In this vector system, the村學 coding regions for heavy and l務體ight chains are included within 醫外a single plasmid separated快什 by a polyadenylation signal吃為. The transcription of the h子少eavy and light cha來間ins is respectively driven by se鐵門parate promoters. In the vector design 喝人process, by customizing sequence微男s encoding the heavy chain and l店姐ight chain varia裡站ble regions, customers may 區河generate vectors to sat火玩isfy their perso店訊nalized need for antigen-binding 森為specificities. The heavy and行話 light chain constant快公 regions may either be selected from 還劇our popular constant region dat街那abase (containing human and mouse IgG作弟1 and mouse IgG2a and 2b heavy cha國和ins; and containing human and mou能市se κ and human and 志藍mouse λ2 and mouse λ1 light cha飛大ins) or be pasted by cust看請omers using their own se我書quence. Additional煙員ly, signal peptides (e.g., IL-2 si請為g) can be added into the vector to i訊放ncrease secretion of the r多關ecombinant antibody.

For further information ab計森out this vector system有去, please refer 船刀to the papers below.