元件指南

VectorBuilder提供了許多常用的載體元件，可體有供用戶在設計載體時(shí)進(jìn)行選擇。下湖冷表按類型提供了這(zhè)些常用元件的詳雪書細信息。
常用shRNA
姓名靶序列Target GeneTarget PositionReferences序列
Scramble[shRNA#1]CCTAAGGTTAAGTCGCCCTCGNone in human and mouseCancer Res. 66:9270 (2006) View
Scramble[shRNA#2]CAACAAGATGAAGAGCACCAATurboGFP; none in 人有human and mouseOncogene. 30:1551 (2011); Science. 343:309 (2014) View
EGFP[shRNA#1]CGACGTAAACGGCCACA麗業AGTTEGFP63-83The RNAi Consortium ID: TRCN000007219裡輛3 View
EGFP[shRNA#2]ACGTCTATATCATGGCCGACAEGFP449-469The RNAi Consortium ID: TRCN00002317黃不56 View
LacZ[shRNA#1]GTCGGCTTACGGCGGTGATTTLacZ1734-1754The RNAi Consortium ID: TRCN0000072242老事 View
LacZ[shRNA#2]GTTCCGTCATAGCGATAACGALacZ1908-1928The RNAi Consortium ID: TR時船CN0000072238 View
Luciferase[shRN線家A#1]ATGTTTACTACACTCGGATATFirefly luciferase還些745-765The RNAi Consortium ID報村: TRCN0000231737 View
Luciferase[shRNA#2]TGTCCGGTTATGTAAACAATCFirefly luciferase1193-1213The RNAi Consortium ID: TRCN00000放生72258 View
Scramble[miR30-shRNA#1]ACCTAAGGTTAAGTCGCCCTCG森姐None in human and mous樹車eDesigned by VectorBuilder View
Scramble[miR30-shRNA#2]CCAACAAGATGAAGAGCACCAATurboGFP; none in human and mouse鐵如Oncogene. 30:1551 (2011); Science. 343:309 (2014) View
EGFP[miR30-shRNA#1]GCGACGTAAACGGCCACAAGTT什機EGFP62-83Designed by Vect看冷orBuilder View
EGFP[miR30-shRNA#2]AACGTCTATATCATG上鐘GCCGACAEGFP448-469Designed by VectorBuilder View
EGFP[miR30-shRNA#3]AAGAACGGCATCAAGGTGAACTEGFP475-496Designed by VectorBui這男lder View
EGFP[miR30-shRNA#4]CACATGAAGCAGCACGACTT拿兒CTEGFP232-253Designed by VectorBuilder View