元件指南

VectorBuilder提供了許多常用的載體元制金件，可供用戶在設計載體時(shí)進(j風舞ìn)行選擇。下表按類型提供了這(zhè)關雪些常用元件的詳細信息。
Regulatory Elements
姓名描述Application NotesReferences序列
WPREWoodchuck hepatiti弟快s virus posttranscriptional regulatory 靜坐element Enhances virus stab民間ility in packaging cells, lea銀人ding to higher titer of packaged viru會文s; enhances highe醫計r expression of tra術理nsgenes.J Virol. 73:2886 (19費了99); Hum Gene Ther. 10:2295 (1999) View
WPRE3Truncated short version of woodchuc計城k hepatitis virus posttrans慢司criptional regulatory elemen輛相t (248 bp)Contained only minima校如l gamma and alpha subunits of WPRE. 做男Slightly weaker effective compare車到d to WPRE.Mol Brain. 7:17(2014); Nat Biomed Eng. 6:1272 (2022) View
oPREOptimized and truncated ve師學rsion of woodchu美長ck hepatitis virus posttran民中scriptional regulatory elementThe potentially oncogenic能上 X protein ORF and 車路X protein promoter have been 腦湖removed; ATG star生作t sites in ORFs encoding費雨 sequences greater than 25 amino 關風acids in length have be地分en mutated.Gene Ther. 13:641 (2006); Hum Gene Ther Me唱師thods. 23: 73 (2012) View