元件指南

VectorBuilder提供了許多常用計得的載體元件，可供用戶在設計載體時(shí)進(分的jìn)行選擇。下表按類型提供了這(zhè)些常用元問空件的詳細信息。

姓名	引導序列	Target Gene	Target Position	References	序列
Scramble[gRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and m紅錯ouse		Engineered by VectorBuilder	View
Scramble[gRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by VectorBuilder	View
Scramble[SagRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mouse		Engineered by VectorBuilder	View
Scramble[SagRNA#2]	GTTCAGGATCACGTTACC他些GC	None in human and mouse		Engineered by VectorBuilder	View
Scramble[msgRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mous近地e		Engineered by Vect相問orBuilder	View
Scramble[msgRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by VectorBuilder	View
EGFP[gRNA#1]	GGGCGAGGAGCTGTTCACCG	EGFP	12-31	Science. 343:84 (2014)	View
EGFP[gRNA#2]	GAAGTTCGAGGGCGACACCC	EGFP	339-358	Science. 343:84 (2014)	View
ROSA26[gRNA#1]	CGCCCATCTTCTAGAAAGAC長麗	Mouse ROSA26 locus	1st intron	Designed by VectorBuilder	View
AAVS1[gRNA#1]	GGGGCCACTAGGGACAG紅什GAT	Human AAVS1 locus	1st intron	Science. 339:823 (2013)	View
EMX1[SagRNA#1]	GGCCTCCCCAAAGCCTGGCCA	Human EMX1	3rd exon	Engineered by Vector微門Builder	View
Scramble[msSagRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mouse		Engineered by VectorBuilder	View
Scramble[msSagRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by Vec知喝torBuilder	View
ttTi5605[gRNA#1]	GATATCAGTCTGTTT票一CGTAA	C. elegans ttTi5605 locus	Adjacent to the但關 ttTi5605 Mos1 inser學嗎tion site on chromosome II	Nat Methods. 10:1028 (2013)	View