元件指南
VectorBuilder提供了許多常用的載體元件,可供用戶在設計載體中坐時(shí)進(jìn)行選擇。下表按類型提湖文供了這(zhè)些常用元件的詳細信息。
哺乳動物廣泛性啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
CMV | Human cytomegalovirus immediate e術舊arly enhancer/promoter | Strong promoter; may have variable st謝雜rength in some c你雨ell types. | Cell. 41:521 (1985); PLoS One. 5:e10611 (201還熱0) | View |
CMV+intron | Human cytomegalovirus immediate early 黃紅enhancer/promoter fused with the splic師麗ing signal from 什也the human beta-globin intron 2 | Strong promoter; may h黑靜ave variable strength in so你村me cell types; presence of the 多事beta-globin intr從微on facilitates the機城 nuclear export of歌說 mRNA by splicing and 日快is predicted to enhance gene expressio裡暗n in eukaryotes. | EMBO J. 13: 3065 (1994); Proc Natl Acad Sci U S A玩資. 105: 3386 (2008) | View |
EF1A | Human eukaryotic trans雪時lation elongation factor 1 α1司多 promoter | Strong promoter. | Nucl Acids Res. 18:5322 (1990下線); PLoS One. 5:e10611 (2010) | View |
EFS | Human eukaryotic tra工和nslation elongat和厭ion factor 1 α1 short fo訊金rm | Medium-strength promoter. | Nat Methods.11:783 (2014) | View |
CAG | CMV early enhancer fuse舞友d to modified chicken β短妹-actin promoter | Strong promoter. | Gene. 108:193 (1991); PLoS One. 5:e10611 (2010) | View |
CBh | CMV early enhancer fused to modi市門fied chicken β-actin promoter | Strong promoter. | Hum Gene Ther. 22:1143 (2011) | View |
CBA | CMV early enhancer fu雨好sed to chicken β-ac雪個tin promoter and SV4錯微0 intron | Strong promoter. | Mol Biotechnol. 58:30 (2016) | View |
SFFV | Spleen focus-forming virus promoter | Strong promoter; drives時黃 high levels of gene expres票見sion, particularly in 問新cell types of the myeloid議間 lineage. | J Virol. 69:7541 (跳長1995); Hum Gene Ther.13:803 (2002) | View |
MSCV | Murine embryonic stem ce醫間ll virus promoter including the e什街nhancer and promoter姐師 region of PCMV virus and 5做飛' untranslated region of雪山 dl-587rev retroviru年從s | Medium-strength promoter; drives gene 都麗expression in most murine or human制用 ES cell lines and other歌筆 mammalian cell lines. | Proc Natl Acad Sci U S A. 87:答南9202(1990); Gene Ther. 1:136 (1994) | View |
MNDU3 | The U3 region from the myeloprolif章作erative sarcoma v藍飛irus (MPSV) LTR with the negative cont音東rol region (NCR) removed | Medium-strength promoter; drives retrov刀對iral vector gene expressio爸學n in most murine o跳服r human embryonal carcin舊劇oma (EC) cell lines, embryonic stem (ES電舞) cell lines and other mammalian cell哥離 lines. | J Virol. 69:748林學 (1995); J Virol. 71: 9466 (1997); J Virol. 77:9439 (2003)讀問 | View |
SV40 | Simian virus 40 enhancer/early promot文報er | Medium-strength 吃又promoter. | Nature. 290:304 (1981); PLoS One. 5:e10611 (2010) | View |
mPGK | Mouse phosphoglycerate k一遠inase 1 promoter | Medium-strength promoter. | Gene. 60:65 (1987); PLoS One. 5:e10611 (2010空動) | View |
hPGK | Human phosphoglycerate kinase 工醫1 promoter | Medium-strength promot志知er. | PLoS One. 4:e5046 (2009) | View |
UBC | Human ubiquitin學廠 C promoter | Weak promoter. | Nucl Acids Res. 24:術自1787 (1996); PLoS One. 5:e10611 (2010) | View |
注意
:
- Some components have different versions媽紅 that differ slightly in s討如equence but are f制數unctionally equivalent說個. VectorBuilder may use dif師拿ferent versions de樹件pending on context.
- CAG and CBh promo友計ters contain different versions o樹黃f modified chicken β-acti民懂n promoter.
哺乳動物組織特異性啓動子
姓名 | 描述 | Tissue Specificity | Cell Type Specificity | References | 序列 |
---|---|---|---|---|---|
Nanog | Mouse nanog prom區筆oter | Embryonic inner cell mass | Pluripotent stem cells such as embr金生yonic stem cells | J Biol Chem. 280:24731 (2005) | View |
Nes | Rat nestin intron 2 enhancer fused 草煙to mouse Hsp68 minimal promoter | Nervous system at embryonic sta雨她ge | Neural stem/progenitor c光地ells | Eur J Neurosci. 9:452下得 (1997); J Neurosci Res. 59:321 (2到這000) | View |
Tuba1a | Rat α1A tubulin prom高校oter | Nervous systems at embryonic and ear友兵ly postnatal stages | Developing neurons but not astroc嗎術ytes or oligodendrocytes | J Neurosci. 14:7319 (1994) | View |
Camk2a(long) | Mouse α-calcium-calmodulin depen民做dent kinase II promoter (8 kb) | Forebrain | CA1 pyramidal cells of嗎在 the hippocampus | Cell. 81:891 (1995); Cell. 87:1317 (199外農6) | View |
Camk2a(short) | Mouse α-calcium火村-calmodulin dependent kinase II promote呢爸r (1.3 kb) | Brain | Pyramidal neurons | Neuroscience. 161:441 (2009) | View |
SYN1 | Human synapsin I promoter | Brain | Mature neurons | Mol Ther. 5:509 (2002) | View |
MIR218 | Human motor-neuron speci著土fic microRNA-218 promoter | Spinal cord | Motor neurons | Science. 339:819 (2013); US11642423B2 | View |
Hb9 | Mouse Hb9 enhancer fused to mous生船e Hsp68 minimal promoter | Spinal cord | Motor neurons | Exp Neurol. 196:224 (2005); Dev Biol. 283:474 (2005) | View |
Th | Mouse tyrosine hydroxylase p紙們romoter | Brain | Dopaminergic neurons | Biochem Biophys Res Commun. 274:5綠路90 (2000); Neurosci Lett. 363:33 (2004) | View |
Thy1 | Mouse thymus cell ant醫河igen 1 promoter | Brain | Neurons | EMBO J. 9:833 (1990); Neuron. 28:41 (2000); Cell Rep. 13:1989 (2015) | View |
NSE | Rat neuron-specif文快ic enolase promot物明er | Nervous system | Various neurons | Neuron. 5:187 (1990); J Mol Neurosci. 8:63(1愛兒997) | View |
GFAP(long) | Human glial fibri舊門llary acidic protein promoter (2.1裡讀 kb) | Brain | Astrocytes | J Neurosci. 14:1030 (1店吧994) | View |
GFAP(short) | Human glial fibril爸舊lary acidic protein pr唱校omoter (0.68 kb) | Brain | Astrocytes | Glia. 56:481 (2008) | View |
Iba1 | Mouse ionized calc刀土ium binding adapt弟線er molecule 1 pr舊窗omoter | Brain | Microglia | J Neurosci Res. 81:357 (2路樂005) | View |
ProA1 | Mouse Gnat2 promoter | Retina | Cone photoreceptor | Nat Neurosci. 22:1345 (2019); Int J Mol Sci. 笑那21:4197 (2020) | View |
ProA7 | Mouse Gnat2 promoter (0.5北熱 kb) | Retina | Cone photoreceptor in non-human prima看得te | Nat Neurosci. 22:1345 (2019) | View |
hRHO | Human rhodopsin pr地拍omoter | Retina | Rod photoreceptor | J Virol. 81: 11372 (2007快她); eLife. 5: e12242 (2016) | View |
hBEST1 | Human bestrophin 1 promo河玩ter | Retina | RPE | Hum Mol Genet. 18:128 (2009) | View |
Grm6 | A 200-bp enhancer of mGluR6 (mous林草e metabotropic glutamat金筆e receptor 6) fused坐坐 to a basal SV40 promoter (0.4 kb劇日) | Retina | ON-bipolar cells | J Neurosci. 28:2空坐008 | View |
Grm6(short) | A 200-bp enhancer of mGlu樂朋R6 (mouse glutamate metabotropic rec門地eptor 6) fused to a 500-bp mGl她可uR6 promoter (0.7 kb) | Retina | ON-bipolar cells | Gene Ther. 23:680 (2016紅內); Proc Natl Acad Sci U S A. 119:e21舊音17038119 (2022)綠在 | View |
Grm6(long) | Intron 4, 3, and a 200-bp e資聽nhancer of mGluR6 (mouse glutamate 購風metabotropic receptor 6) fused to明業 a 500-bp mGluR6 promo不民ter (2.2 kb) | Retina | ON-bipolar cells | Gene Ther. 23:680 (2016); Proc Natl Acad Sci U S A歌兵. 119:e2117038119 (嗎吧2022) | View |
Prnp | Mouse prion protein p線鐵romoter | Brain, hippocampus, cerebellum, reti秒不na | Various CNS neu懂術rons | Eur J Neurosci. 14:1777 (2001) | View |
Cnp | Mouse 2′,3′-cyclic nucleotide 3′-現熱phosphodiesterase promoter | Nervous systems, testis and thy這看mus | Oligodendrocytes and Schwann 算厭cells | J Neurosci Res. 53:393 (199靜場8); J Neurosci. 19:759 (1999) | View |
K14 | Human keratin 14 promoter | Epidermis | Keratinocytes | Gene. 153:297 (1995) | View |
K19 | Mouse keratin 19 promoter員物 | Epidermis | Keratinocytes | J Biol Chem. 275:28230 (2000劇長); Genesis. 46:318 (2008) | View |
BK5 | Bovine keratin 靜件5 promoter | Epidermis | Keratinocytes | Differentiation. 58:朋人53 (1994); Oncogene. 19:4243 (2000) | View |
mTyr | Mouse tyrosinase promoter and 事秒enhancer | Epidermis | Melanocytes and 友看melanoma cells | Development. 120:2103 書月(1994); Gene Ther. 1:307 (1994); Int J Mol Sci. 17: 2149 (2016)你關 | View |
cTnT | Chicken cardiac troponin T promoter | Heart | Cardiomyocytes | Am J Physiol Cell Physiol. 286:C畫習556 (2004); Gene Ther. 18: 熱姐43 (2011); Circ Res. 115:3畫村54 (2014) | View |
αMHC(long) | Mouse α-cardiac my商用osin heavy chain promot身筆er (5.4 kb) | Heart | Cardiomyocytes | J Biol Chem. 266:246拍視13 (1991) | View |
αMHC(short) | Mouse α-cardiac myosin heavy cha內子in promoter (2.8 kb) | Heart | Cardiomyocytes | J Biol Chem. 26藍民6:24613 (1991) | View |
Myog | Mouse myogenin prom近喝oter | Muscle | Myoblasts | Genes Dev. 7:1277 (1993) | View |
ACTA1 | Human skeletal muscle actin α1 pro慢用moter | Muscle | Myocytes | J Biol Chem. 268:放麗719 (1993) | View |
MHCK7 | Composed of enhancer/promoter regions o她物f murine muscle creatine kinase (MCK)訊他 and enhancer reg也哥ion of α-myosin heavy-chain ge朋計nes | Mouse skeletal and heart muscles | Differentiated postmitotic striated mu我錯scle cells | Mol Ther. 15:320 (2007); Sci Transl Med. 9(418) (2厭村017) | View |
CK8e | Muscle-specific creatine kinase 信大promoter with deletion of poorly c唱讀onserved regions | Mouse skeletal and heart muscle | Differentiated postm術秒itotic striated muscle c樂裡ells | Mol Ther. 19: 1331 (201明些1); Acta Naturae. 13: 47匠河 (2021) | View |
SPc5-12 | Synthetic promoter c5-12 cont的體aining a combination of muscle spec上跳ific transcription factor binding sites謝科 SRE, MEF-1, MEF-2 and TEF-1 | Mouse skeletal, hear自姐t, and smooth muscle | Differentiated postmitotic striated 對會muscle cells | Nat Biotechnol. 17:241 (1999); Acta Naturae. 13:刀文 47 (2021) | View |
SM22a | Mouse transgelin (a.k.a. Sm22a) prom腦國oter | Adult smooth muscle | Vascular smooth 低月muscle cells | J Cell Biol. 132:849 (1996) | View |
EnSM22a | The chimeric vascular smooth muscle-sp下如ecific enhancer/promoter 廠歌(The rabbit myosi男嗎n heavy-chain enhancer inse了身rted upstream of a truncated mouse SM22厭影a promoter) | Adult smooth muscle | Vascular smooth muscle cells | J Pharmacol Exp Ther. 329: 775線訊 (2009) | View |
Runx2 | Mouse runt related transc了男ription factor 2 pr業麗omoter | Bone | Early osteoblasts | Biochim Biophys Acta.開秒 1731:95 (2005) | View |
OC | Human osteocalcin promoter報訊 | Bone and cartilage | Osteoblasts, osteocytes 刀少and hypertrophic鐵城 chondrocytes | Mol Endocrinol. 11:1695麗書 (1997) | View |
Col1a1 | Rat collagen type I α1 promoter | Bone, tooth and tendon | Osteoblasts and fibroblasts | J Bone Miner Res. 9:285 體窗(1994) | View |
Col2a1 | Mouse collagen type II α1 promot場機er | Cartilage | Chondrocytes | Genesis. 45:44 喝要(2007) | View |
aP2 | Rat adipocyte P2可志 promoter | Fat | Adipocytes | J Biol Chem. 268:22243 (1993) | View |
Adipoq | Mouse adiponectin promoter | Fat | Adipocytes | Endocrinology. 151:2933很西 (2010) | View |
Tie1 | Mouse tyrosine k分下inase with immunoglob笑錢ulin-like and EGF-like d還樹omains 1 promoter | Blood vessel | Endothelial cells | J Cell Sci. 115:207不微5 (2002) | View |
Cd144 | Mouse cadherin 5 (a.k.a. VE-Cadherin) 東遠promoter | Blood vessels | Vascular endothelial cells | Dev Dyn. 235:759 (2006)行湖 | View |
CD68(short) | Human CD68 promoter, short, recommen資他ded for in vitro us北信e | Blood | Monocytes and macrophages | Gene Ther. 19:1041 (2012)空議 | View |
CD68(long) | Human CD68 promoter, long窗筆, recommended for in vi玩好vo use | Blood | Monocytes and macrophages | J Immunol. 168:3402(2002); Blood. 124:e33 (2什又014) | View |
CD11b | Human integrin subunit alpha M p畫林romoter | Bone marrow | Mature myeloid cell lines (neutrophils,近下 monocytes and macrophages) | Blood. 79:865 (1樂大992); Blood. 85:319 (1995) | View |
Afp | Mouse α-fetoprotein en飛什hancer II fused with human beta glo拍船bin promoter | Liver | Hepatocytes | Science. 235:53 (1987); Mol Cell Biol. 15:4947 (1995) | View |
Alb | Mouse albumin promoter | Liver | Mature hepatocytes | Genes Dev. 1:268 (1987) | View |
TBG | Human thyroxine-binding gl謝吃obulin promoter | Liver | Hepatocytes | Gene. 506:289 (2012) | View |
TBG+intron | Human alpha-1-mic你報roglobulin/bikunin enhance快高r and thyroxine-binding globuli門些n hybrid promoter fus公讀ed to a chimeric intron (SD s醫腦ite from a human β-數白globin intron and SA site from an immun房白oglobulin heavy chain vari錢他able region intron) | Liver | Hepatocytes | J Biol Chem. 267:20765 (1992); Nat Chem Biol. 17:326 (2021) | View |
MMTV | Mouse mammary tumor virus long term公做inal repeat (LTR腦票) | Mammary gland | Ductal cells of t如鄉he salivary gland, mammary epi近著thelial cells | Nucleic Acids Re拍也s. 25:4323 (1997) | View |
Wap | Mouse whey acidi影科c protein promo見黃ter | Mammary gland | Alveolar epithelial cells of mamma能腦ry tissue | Nucleic Acids Res. 25:4323 (中放1997) | View |
HIP | Human insulin promoter | Pancreas | β cells | Cell. 52:773 (1998); Proc Natl Acad Sci U 高件S A. 100: 6688 (2003) | View |
Pdx1 | Mouse pancreatic and duod大村enal homeobox 1 promoter | Embryonic developing pancrea黑街s and adult pancreatic i坐飛slets | Pancreatic progenitor cells and pancre開說atic β cells | Development. 129:2447 (2002) | View |
Ins2 | Rat insulin 2 p中從romoter | Pancreas | Pancreatic β cells | Nature. 315:115 (1985);Nature. 429:41 (2004) | View |
Hcn4 | Mouse hyperpolarizati坐坐on-activated cyclic nucleotide-gate快視d K+ 4 promoter | Embryonic heart | Cardiomyocytes | Biochem Biophys Res Commun. 353:67 (200她訊7) | View |
NPHS2 | Human podocin prom懂照oter | Kidney | Podocytes | Genesis. 35:39 (2003) | View |
SPB | Human surfactant protein B也去 promoter | Lung | AT II cells (alveolar type II epi綠什thelial cells) and資光 Clara cells (bronchiolar epithelia和銀l cells) | J Biol Chem. 270:24852 (1995); Gene Ther. 14:1461 秒煙(2007) | View |
CD144 | Human cadherin 5 (a.k.a. VE-C亮還adherin) promoter | Lung, heart, ovary, spleen and kidney 場道glomeruli | Vascular endothelial cells | Oncogene. 24:2992 (2005) | View |
TERT | Human telomerase reverse t吃又ranscriptase promoter | Tumor | Immortalized and cancer c窗制ells | Cancer Res. 59:551 (1999); Cancer Res. 60:5359 (2000河志) | View |
哺乳動物誘導型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
TRE | Tetracycline-responsive element promo為地ter (2nd generation) | Regulated by a class of t物小ranscription factors (e.g. tTA, rtTA an書人d tTS) whose activities are depen亮中dent on tetracycline 看器or its analogs (e.g. doxycycline)哥對. | Proc Natl Acad Sci U S A. 89:5547 (19南事92); Science. 268:1766 (1995); J Gene Med. 1:4 (1999) | View |
TRE3G | Tetracycline-responsive 高那element promoter 相聽(3rd generation) | Bound by transactivator Tet3G in th明分e presence of tetracycline 會黑or its analogs (e.g. doxycycline). | Gene Ther. 13:1382 (2006) | View |
酵母誘導型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
GAL1 | Saccharomyces cerevisiae ga從人lactokinase promoter | Repressed in the presence of gluc火答ose; induced in the presence o黑影f galactose. | Mol Cell Biol. 不長4:1440 (1984); Nucleic Acids Res. 22:5物錯767 (1994) | View |
MET17 | Saccharomyces cerevi還市siae O-acetyl homo區有serine-O-acetyl serine sulfhyd紅事rylase promoter; aka MET2線照5 and MET15 | Repressed in the pr高身esence of methionine. | Mol Gen Genet. 200老笑:407 (1985); Biotechnol Prog. 21:617 (2不自005) | View |
CUP1 | Saccharomyces cerevisiae 玩黑metallothionein promoter | Induced in the presence of coppe亮黃r. | Proc Natl Acad Sci U S A 81:來玩3332 (1984); Yeast. 16:651 (光對2000) | View |
AOX1 | Methanol-induced alcohol oxidase 外不1 promoter from Pichia pastoris | Maximally induced during growth on火多 methanol and full靜鐵y repressed during growt一作h on glucose or glycerol. | Nucleic Acids Res. 15:3859 (198說月7); J Ind Microbiol Biotech區呢nol. 42:1175 (2015兒讀) | View |
斑馬魚廣泛性表達啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
sCMV | Simian cytomegalovirus immediate家票 early enhancer/promoter | Strong promoter; Can function in Xenop謝低us, zebrafish, mouse and 照師human. | Gene Expr Patterns. 9:54 (文學2009); Biotechnol Lett. 33:1319 (2011)業南 | View |
bactin2 | Zebrafish cytoskeleta議醫l beta-actin promoter | Strong promoter. | Dev Biol. 192:289 (1997) | View |
Ubi | Zebrafish ubiquitin (ubi) promoter | Ubiquitous and constitutive pro文資moter. | Development. 138:169 (2011) | View |
斑馬魚組織特異性啓動子
姓名 | 描述 | Tissue specificity內子 | Cell type specificity | References | 序列 |
---|---|---|---|---|---|
cmlc2 | Cardiac myosin light chain 2 p開房romoter | Heart | Cardiomyocytes | Dev Dyn. 228:30 (2003); Dev Cell. 32:756 (2015) | View |
zK5 | Zebrafish Keratin 5 pr媽資omoter | Tongue and fin | Squamous epider舞畫mis | Oral Oncol. 46:31 (2010); PLoS One. 9:e92217 (2生飛014) | View |
503unc | 503-bp promoter 靜店of zebrafish unc-45b | Muscle | Myoblasts | Genesis. 51:443 (2013)土多 | View |
斑馬魚誘導型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
zHSP70 | Zebrafish heat-shock protein 照紅70 promoter | Strong and heat-shock愛信 inducible promo工車ter. | Development. 127:1953 (2拍玩000); Nat Methods. 7:219 (201腦制0) | View |
5×UAS | Five tandem upstream activation sequenc畫坐e (UAS) followed by E1B 湖林minimal promoter | Activates gene transcr筆樂iption upon binding with GAL4 prot吃我ein. | Cell. 52:169 (1988); Nat Methods. 7:219 (2010) | View |
植物組成(chéng)型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
CaMV35S(short) | Cauliflower mos看站aic virus 35S promote吧廠r (short) | Strong constitutive p化雜romoter, drives店電 high-level gene expr看美ession in dicot plants but筆器 is less effective in monocots們子. | Nature. 313:810 (1985) | View |
CaMV35S(long) | Cauliflower mosaic virus得到 35S promoter (long市熱) | Strong promoter計吃. | Biotechnol Lett吧就. 29:1793 (2007) | View |
CaMV35S(enhanced) | A chimeric promoter with the doubled C姐藍aMV 35S enhancer element | Strong promoter. | Plant Mol Biol. 國校21:415 (1993); Biotechnol Lett. 29:17店自93 (2007) | View |
Nos | Agrobacterium nopaline 輛子synthase promoter | Weaker promoter compared to 湖藍CaMV 35S promoter. | Nucleic Acids Res. 12:7831 (1984); Plant Cell. 2:225 (1990) | View |
ZmUbi | Maize ubiquitin promoter | Strong constitutive promoter, drive和對s high-level gene expressio友購n in monocots. | BMC Plant Biol. 14: 327 (2014) | View |
酵母組成(chéng)型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
TEF1 | Saccharomyces cerevisiae transl時費ation elongation factor EF-1 a匠站lpha promoter | Strong promoter. | EMBO J. 3:3311 現這(1984); Yeast. 27:955 (2010) | View |
GPD | Saccharomyces cerevisiae glyceral匠著dehyde-3-phosphate dehydroge資美nase promoter | Strong promoter. | Gene. 32:263 (1984可美) | View |
ADH1(short) | Saccharomyces c志草erevisiae alcohol dehydrogenase 村睡promoter | Weak promoter (stronger than ADH1(m睡黑edium) and ADH1(long)). | FEMS Microbiol Lett. 121:22腦道9 (1994) | View |
ADH1(medium) | Saccharomyces cerevis票票iae alcohol dehydrog知明enase promoter | Weak promoter (st業刀ronger than ADH1(long)). | FEMS Microbiol Lett. 121:22知媽9 (1994) | View |
ADH1(long) | Saccharomyces cerevi自低siae alcohol dehydrogena懂著se promoter | Weak promoter. | FEMS Microbiol Lett. 12睡姐1:229 (1994) | View |
GAP | Glyceraldehydes-3-p黃做hosphate dehydrogenas國工e promoter from Pichia 廠慢pastoris | Strong constitutive promoter. | Gene. 186:37 (1997); J Ind Microbiol Biotech書土nol. 42:1175 (2015)玩日 | View |
果蠅廣泛性表達啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
actin5C | The distal promoter of花新 cytoskeletal actin 5C gene o來員f Drosophila melanogast公個er, controling s玩低ynthesis of actin in a 音文tissue- and developmental 訊公stage-specific m開友anner | Strong promoter | Mol Cell Biol. 10:上友6172 (1990); Nucleic Acids Res. 19:5037業光 (1991) | View |
Polyubiquitin | Promoter of pol舞事yubiqutin gene of 做計Drosophila melano路亮gaster | Strong promoter | Mol Cell Biol. 8:4727 (1雜答988); Methods Mol Biol. 62:385年睡 (1997) | View |
a1tubulin | Promoter of α1-tubulin gene of 紙相Drosophila melanogaster | Medium-strength p錢司romoter | Nucleic Acids Res. 19:山木5037 (1991) | View |
果蠅組織特異性啓動子
姓名 | 描述 | Tissue specificity | Cell type specificity | References | 序列 |
---|---|---|---|---|---|
Rh2 | Promoter of Rh2 gene of Drosophila 現暗melanogaster | Ocelli | Ocellar photoreceptor cell舞喝s | Genetics. 120:173 (1988) | View |
果蠅誘導型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
Mtn | Promoter of the metallothionein物村 gene of Drosophila melanogaster | Strong promoter; tightly ind道妹ubible with the presence of Cu+. | Nucleic Acids Res. 19:503雨市7 (1991); Genetics. 112:493 (1樹湖986) | View |
DmHsp70 | Drosophila melanogaster he吧不at-shock protein刀白 70 promoter | Strong and heat-shock inducible promo計對ter. | BMC Evol Biol. 13:68 (2013); Plos one. 10:e0128976歌懂 (2015) | View |
果蠅Pol III啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
U6-1 | U6 small nuclear RNA gen科可e promoter U6-1 of Droso慢輛phila melanogaster | Pol III promoter; drives in畫知termediate expression level of畫煙 small RNAs. | Biochem Biophys Res Commun. 331:1163 (2家愛005); Proc Natl Acad Sci U S A. 111:E2967 (20通務14) | View |
U6-2 | U6 small nuclea看城r RNA gene promote友草r U6-2 of Drosophila melanogaste刀吃r | Pol III promoter; drives務們 lowest expression土見 level of small RNAs. | Biochem Biophys Res Commu線學n. 331:1163 (2005)行在; Proc Natl Acad 又匠Sci U S A. 111:E2967 (2月店014) | View |
U6-3 | U6 small nuclear RNA gene promoter U6-3妹吃 of Drosophila melanogaster | Pol III promoter; drive藍員s strongest expression level of small 分習RNAs. | Biochem Biophys Res Commun. 3票腦31:1163 (2005); Proc Natl Acad Sci U S車房 A. 111:E2967 (2014)白物 | View |
植物Pol III啓動子
姓名 | 描述 | Application Not體事es | References | 序列 |
---|---|---|---|---|
AtU6-26 | Arabidopsis U6-26 gene promoter | Pol III promoter; drives exp看書ression of small RNAs. | Journal of Integrative Plant Biology. 4飛銀9: 222 (2007); Cell Res. 23: 1229 (2013) | View |
OsU3 | Rice U3 promoter | Pol III promoter; drives expre筆靜ssion of small RNAs. | Nat Biotechnol. 31:686 (2013); Sci Rep. 6: 21451 (2016) | View |
線蟲廣泛性啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
eft-3 | C. elegans translation elonga爸要tion factor 1-α pr錢見omoter | Strong promoter;是能 drives high levels of gene expression坐遠 in somatic cells. | Genes Dev. 14: 2173 (2000) | View |
pie-1 | C. elegans pharynx and intestine嗎少 in excess protein 1 pr花紙omoter lacking rep紙新etitive sequences | Strong promoter; drives high level船場s of gene expre還習ssion in all germ cells街視 from the late L1 stage to the adu票到lt stage. | Curr Biol. 18:1476 (2008) | View |
CeU6 | C. elegans R07E5.16 U6不司 snRNA promoter | Pol III promoter; drives expressi電風on of small RNAs. | Nat Methods. 10: 1火鄉028 (2013); Genetics. 199: 959 (2015) | View |
線蟲組織特異性啓動子
姓名 | 描述 | Tissue Specificity | Cell Type Specific森頻ity | References | 序列 |
---|---|---|---|---|---|
spe-11 | C. elegans spermatocyte prot遠妹ein spe-11 promoter | / | Sperm | Curr Biol. 18:1476 (2008)區和 | View |
myo-2 | C. elegans myosin-2 pr亮刀omoter | / | Pharyngeal muscle cells | Genetics. 135:38子動5 (1993); Nat Genet. 40:1375 (2008) | View |
myo-3 | C. elegans myosin-3 promoter呢還 | / | Body muscle cells | Genetics. 135:385 (1993); Nat Genet. 40:1375靜水 (2008) | View |
rab-3 | C. elegans ras-r男志elated protein Rab-3 prom鄉分oter | Nervous system | Pan-neuronal | Proc Natl Acad Sci U S作低 A. 113: E1074 (2016) | View |
線蟲誘導型啓動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
hsp16-2 | C. elegans heat s舞間hock protein Hsp-16.2 promot低區er | Strong and heat-sh從裡ock inducible promoter in劇離 most somatic c妹分ells. | Genome. 31:690 (198暗做9); J Cell Biol. 200: 5喝子89 (2013) | View |
hsp16-48 | C. elegans heat shoc是謝k protein Hsp-16.48 promo紅謝ter | Strong and heat-shock生答 inducible promoter in 上厭somatic cells and germline. | Nature. 413:70 (2001); Nat Genet. 40: 1375 (2008) | View |