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向(xiàng)使用過(guò)VectorB年鄉uilder産品和服務的客戶表示感激。你們的滿意,才是我們鞠躬盡子藍瘁的動力。在此,很榮幸分享你們的感謝信。謝謝!
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Amazing experience with 笑技VectorBuilder! Ordered plasmids beginni內慢ng of December, and despite 你金the holiday season they arrived e車花xactly as scheduled. Furthermore, cus討店tomer support reached out to a金水sk what would be my desire家服d delivery date, considering pot空習ential holiday time at my institute姐很. Overall, I am su輛器per satisfied, would highly recommend 章文VectorBuilder (which I already do) and玩那 will buy from them again. All街河 the best from my side!舞唱
Tamara Tomin
Medical University of Graz, Aus錢愛tria
We began to use VectorBuilder to p業一repare adenoviral plasmids about a year從有 ago. We normally handled all爸街 the cloning stuffs ourselves女要, but when it comes to adenoviral v風請ectors, because of its large size, so線多metimes it is kind of tricky for ine農報xperienced stud生北ents. We decided to give a try on Vecto老影rBuilder. One month later懂事, the plasmid arrived and it generated哥放 virus that expressed the co城白rrect gene. After that, w票做e have ordered more than 10 adenoviral 間場plasmids and they all work fin答學e. VectorBuilder did save u錯慢s a lot of time.
Lei Sun
DUKE-NUS Graduate 房輛Medical School, Singapore
I ordered a plasmi國關d vector two years 愛水ago. The design on the web platform was要一 easy. Contacts with t劇件he specialists to arran土電ge further details were ex南購cellent: they are very supportive!
Emanuela Signori
Consiglio Nazionale delle Ricerche資熱, Italia
Using VectorBuilder has transformed南技 the way I do my research. Ve開飛ctorBuilder’s easy-t醫務o-use web interface a去信llows me to custom b為票uild my own plasmids, freeing u筆門p time that I would have wasted clo算通ning and instead letting me信靜 focus on the research that rea生林lly matters. They offer 爸近great prices with a fast turnarou又是nd time. Their friendl市秒y and well-informed technical s人門upport team are on hand遠那 every step of the way, s知懂hould you need any help. I would物習 strongly recommend Vec訊車torBuilder to anyone 線又wanting to accelera討算te the progress 答文of their research.
David M Brown
University of Nott老妹ingham, UK
We recently purchased from VectorBu大但ilder a lentiviral plasmid for constit資亮utive expressio男習n of GFP and luciferase and the co在音rresponding lentivirus. We had inf報業ected several tumor cel紙多l lines with excellent results. 南得The lentiviral stocks音這 worked very nice sa快拿ving us a lot of time照我. We are now using thes對門e modified cell lines for mo店睡nitoring tumor growth i山醫n animal models using in微紅 vivo imaging.
Javier Alonso
Research institute f謝文or Rare Diseases, Spain
VectorBuilder is 吃高an amazing way to save道制 time on molecular biolog了工y. The web site is easy to use, well黑去-conceived and a黑錢llows quick design in most cases. For m頻我ore unusual requests, the team我影 is energetic, reply promptly on ev作金ery request, and always finds a 睡習way to help. Low pr時黑ices and short timelines really make自視 it possible to consider Vect厭議orBuilder on many cloning 中唱matters.
Laura Israel
Novartis (Basel), Swi媽呢tzerland
We requested a custom AAV (司美4-YF) from VectorBuild大廠er. The ordering process was easy and t了坐he customer support team were reall討從y helpful. Our vir看麗us had the expected titer and worke玩下d extremely well做花 for our chosen 拿離application.
Jessica Rodgers
The University of Manches體影ter, UK
我經(jīng)常使用雲舟生物的載體構建服務,對(duì)于他們的産品質外美量我總體很滿意,我遇到的一些難題他們也能(néng)很快幫助我解決。
Huimin Chen
中山大學(xué)附屬第一醫院火弟
VectorBuilder has provided公店 very satisfactory servic是好e in making various AAV vector const相他ructs for us. The new sal身見es representative is very proactive著男 in offering help on expla場金ining and introducing other VectorB廠吧uilder service to us. Ex林技pecting to get more suppor東北t and service f山訊rom VectorBuilder.
Qingchun Tong
University of Texa山光s Medical School at H業聽o, USA
I have been consistently using Ve紅又ctorBuilder over the爸村 last couple years and hav算歌e been extremely satisfied with th這中e vectors delivered to 笑和me. These include standard over-expre章下ssion vectors, and 不南Cas9 vectors on piggybac backbone.房分 All of the vector公放s I have purchased so far ha票鐵ve passed my QC and 數件provided reproducible functional data.長西
Daksh Thaper
Vancouver Prostate 亮國Centre, Canada
I have been successfully clo些我ning for 3+ years, but 音算the last time we tried g少民enerating KO cell lines with CRI著廠SPR/Cas9 vectors, we w物報ere not successful. This was upsettin跳長g, especially since 雨刀I invested a lot of time in these ef拿麗forts. Then, by chance, I attende生如d one of VectorBui秒愛lder's seminars. Due to their reasona匠區ble prices and the fact I could focus 城了my time on other work, we dec內子ided to give their lentivi女雪ral (dual gRNA) Cas9 plasmid a try. Th老煙e turnaround time was re是會asonable, their c水藍ustomer service w是件as excellent and 技機they actually gave feedback abou可還t the status of my cloning projects. 我電Their website is also easy 服山to use. But most importantly什體: using their Cas9 dual
gRNA vector, I was able to successfully 志海generate KO cell lines!I recommend VectorBuilder to CRIS城大PRCas9 users without any 是子hesitation.
Anna Salamero B些如oix
Georg-Speyer-Haus Fran國房kfurt, Germany
雲舟生物爲我的shRNA敲低實驗提供了載體構建和慢病毒包裝服務。他們的慢病到店毒的轉導效率很高,而且員工也非常專業,總能輛鄉(néng)很快回複我的技術問題。在雲舟生物去高幫助下,我很快完成(chéng)了我的實驗,目前已經(jīng)在準都筆備論文投稿的工作。
Mengyuan Fan
北京林業大學(xué)自然保護學(xué)院
We have used VectorBuilder for mul章藍tiple vectors i少喝ncluding regular, lentiviral, Tet-土微inducible, and CRISPR/Cas9 vector相鄉s. Designing the vectors is sup用子er easy and we ha購路ve had good luc男錢k with them. I would definitely recomm工校end their products!
Steven Wall
Texas A&M University, US友近A
Before using Vec她數torBuilder, produci開化ng lentiviral overexpression vec喝票tors for our in vitro model影科s was a recurring and time-co美視nsuming enterpr科她ise. We faced frequent challenges 技站in every experimental ste市線p, which made it difficult to放跳 plan the actual experime冷唱nts. Setting up the 間劇process with VectorBuilder was ver技刀y efficient, thanks to their great e男南xpertise and support. 師鐘Once we started u妹生sing VectorBuilder's services, we n市我oticed a substantial increase in pro器放ductivity as well as數兵 a more uniform and predi就車ctable transducti看風on pattern of our target 司訊cells.
We went on to use Vect朋爸orBuilder for a large-scale CRISPR 都女screen with a cust議放om library spanning over 1司紙,600 genes. With the p對讀rovided virus, we were able to ea年紙sily maintain cell represent要議ation and we de書件rived a number of inter知廠esting hit genes, which we are ex弟計cited to follow up on. A big thank yo知電u to the entire VectorBuilder 物窗Team!
Dominic Schmid
University of Basel
We were searching for a 算機way to outsource the clo懂姐ning of our vectors and 說友we are very happy wit電術h the service. The versa國自tility of the tools make跳有 it a great time saver 木服and the vectors were delive師文red on-time. Lenti藍微viruses packaged ar火快e also very reliable and have been ve窗城ry successful in transduction s雪對tudies in primary cells. G快南reat customer s場很ervice with fast response times and 呢做the team at VectorBuild木鐵er are very knowledgeable.
Kathy Ngo
Aridis Pharmaceuticals, USA
請告知我們您的體驗!
Lis R et al.
Nature. 545: 439 (2017)
Conversion of adult endothelium to im車器munocompetent haemato為上poietic stem cells.
Thomas CA et al.
Cell Stem Cell. 21: 319 (20時城17)
Modeling of TREX1-Dependent Autoi老信mmune Disease using Human Stem Cells H計科ighlights L1 Accumulat路章ion as a Source of Neuroinflamm得問ation.