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I have been working with VectorBui道有lder from the beginning of藍秒 my postdoc. I’ve 裡議chosen VectorBu為哥ilder because of the online platfor雨花m to create customized ve習議ctors. It is really i為醫mpressive how easy is to dig答房itally clone and the化車 order process. Spe鐘她cifically, in our paper, we’雜行ve used VectorBuilder for se相關veral critical 會林experiments, including the 到場downregulation of Bassoon prote體謝in (AAV-shRNA) and to stu一遠dy the role of Bassoon in the pr唱黑opagation of tau (AAV-GFP-P相大2A-hTau). These experiments we務報re extremely important西地 to probe our hypothesis.
Dr. Pablo A. Martínez
Indiana University School of房南 Medicine, USA
I have been using VectorBuilde他到r's cloning service for several years,頻美 and am extremely happy with 和時their products and 答可service. Their online design s費離oftware is very easy to use and prov秒雜ides the most cutting edge so鐘低lutions for today's m得兵ost demanding molecular cloning ne湖能eds. Their customer service is 城場outstanding, with great t放分echnical compet風兒ence and is always 很民on time to delivery 錯你the excellent results, which拍睡 have saved me months of靜熱 precious research tim花器e. I would recommend Vecto問女rBuilder to anyone who事去 want the best vector design and molec雪湖ular cloning ser大服vice.
Guohao Dai
Northeastern Univ分就ersity, USA
The scientific standards of Vecto匠個rBuilder’s services are unque師影stionably high. The web i湖器nterface to design vectors is i區在ntuitive and in 時習fact so easy to use that it唱理 can even serve for edu黃朋cational purposes. Vector玩麗Builder has pretty much covered ev也坐erything one can think about wh醫家en wanting to design輛作 an expression vector, it林這’s great to have all the versat道地ility on one page. Turn道舊around time is fast and owing to their 聽和professionalism and customer fr坐媽iendly attitude I can recomme刀多nd VectorBuilder’s services 女船without reserve.
Johnny Kim
Max Planck Institute for下工 Heart and Lung Rese友喝arch, Germany
VectorBuilder’s service makes des熱電igning vectors i近分ncredibly simple an店這d there’s no software required, 自分it can all be done through their ea區吧sy to use website. The lentivirus p厭一roduction service is a mas員務sive bonus as it’s so quick and reas話有onably priced th讀線ere’s really no reason to make 錢友our own lentivirus anymore. Tha西也nks for a fantas習電tic service.
Timothy London
TC Biopharm, UK
VectorBuilder is an amazing way t日制o save time on molecular biology. 短頻The web site is ea還村sy to use, well-conceived森劇 and allows quick近姐 design in most cases. 錯畫For more unusual requests, the te爸服am is energetic, reply prompt木錢ly on every request, and always finds 資歌a way to help. Low prices and s海校hort timelines re唱離ally make it possible to consider 黑計VectorBuilder on ma章電ny cloning matters.
Laura Israel
Novartis (Basel),體文 Switzerland
I recently met one of the 裡冷VectorBuilder product speci話算alist during AAI-20影冷19 meeting at San Diego Conve一但ntion Center. Her name is Dr. 兒飛Swati Roy, and she provided me 農做excellent information regarding t鐵拍heir products an算錯d vector designs. And then, we recently厭讀 purchased CRISPR Cas9 dual gRNA vec員中tor. Now, we are ordering few mo討我re plasmid vectors. As p你女er my experiences, V學知ectorBuilder customer services are ex廠多cellent and user-friendly. The VectorBu線老ilder product prices are reasonabl老我e.I recently met子那 one of the Vecto裡筆rBuilder product specialist d錢微uring AAI-2019 meeting at San D門路iego Convention Center. He說資r name is Dr. Swati Roy, and動短 she provided me excellent info鄉市rmation regarding their 朋跳products and vector designs. And then,對數 we recently purchased CRISPR 著雨Cas9 dual gRNA vector. 些亮Now, we are ordering few more 哥站plasmid vectors. As per my e開紅xperiences, VectorBui費制lder customer servi嗎金ces are excellent and user-去舊friendly. The VectorBuilder product女市 prices are reasonable.
Shahanshah Khan
UT Southwestern Medical 地機Center, USA
Before using VectorB醫和uilder, producing lentiviral o兵身verexpression vecto是數rs for our in vitro models was a recurr店美ing and time-consuming enterprise. We 坐西faced frequent ch身山allenges in every exper學大imental step, which made it dif拍熱ficult to plan the actual experi費藍ments. Setting up the process wit麗長h VectorBuilder was湖又 very efficient, thanks to 樹男their great expertise and support. Once靜都 we started using VectorBuilder資些's services, we notice民輛d a substantial i東拍ncrease in product靜喝ivity as well as a more uniform and pre亮們dictable transduction pattern o亮計f our target cells.
We went on to use VectorBuilder for a 厭工large-scale CRISPR screen個微 with a custom library spanning ove也錢r 1,600 genes. With the pr見河ovided virus, we w和路ere able to easily maintain cel也湖l representation and we城東 derived a number of interest廠要ing hit genes, which we are ex從睡cited to follow up 煙冷on. A big thank you to the entire Ve購分ctorBuilder Team!
Dominic Schmid
University of Basel
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Lis R et al.
Nature. 545: 439會人 (2017)
Conversion of adult endot工森helium to immunocompetent h做頻aematopoietic stem cells.
Thomas CA et al.
Cell Stem Cell. 21: 319 (2017)
Modeling of TREX1-De喝能pendent Autoimmune Dise校劇ase using Human Stem Cells Highligh東車ts L1 Accumulation as a Source吧動 of Neuroinflammation.