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Before using Vecto間都rBuilder, producing 在城lentiviral overexpression ve時這ctors for our in vitr自裡o models was a recurring a小紙nd time-consuming enterpri木分se. We faced frequent chal那體lenges in every exper火看imental step, which 數新made it difficult to plan the actual 下鐘experiments. Setting up the pr通物ocess with Vector微筆Builder was very efficient, thanks to不煙 their great expertise and support黃家. Once we started using VectorBuil吃相der's services, we noticed a substantia窗紙l increase in prod門得uctivity as well as a more un報地iform and predictable transduction patt得白ern of our target ce自喝lls.
We went on to use VectorBuilde業鐘r for a large-scale CRISPR scr河術een with a custom librar吃在y spanning over 1,600 genes.媽姐 With the provided virus, we we哥看re able to easily maintain還熱 cell representation日一 and we derived a number of inter會業esting hit genes討視, which we are exc什爸ited to follow up on. A big than唱人k you to the en站慢tire VectorBuilder Team!
Dominic Schmid
University of Basel
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Lis R et al.
Nature. 545: 439 吧是(2017)
Conversion of adult en遠市dothelium to immunocompet習費ent haematopoietic stem cells.
Thomas CA et al.
Cell Stem Cell. 21: 319 (2017)到朋
Modeling of TREX1-Dependent Autoimmun藍頻e Disease using Human Stem Cells Highli務亮ghts L1 Accumulat懂老ion as a Source of Ne看湖uroinflammation.