Pheochromocytoma is a rare tumor 很電that develops in the adrenal gland湖理s. To discover nove空從l driver events of the disease,畫我 Dr. Patricia Dahia and her team at低知 the University of Texas San Ant章呢onio Division of Hematology and Medica草都l Oncology conducted whole transcripto西去me RNA sequencing of pheochromocytoma土女 tumors. After 文廠analyzing the data, the team disc雜玩overed a novel fusion betw歌還een RET, a gene草子 commonly mutated in multipl上間e cancers, and GRB2 a kno朋鐘wn adaptor protei她議n and signaling mediator of the銀南 RET protein.

RET is a cellular growth receptor that懂著 is found to be mutated in lung, th木朋yroid, colon, prostate, and breast c朋影ancers. After cond事筆ucting whole transcriptome sequencing 開北of 30 pheochromocytoma 遠體tumors, Dr. Dahia and her group友農 identified a novel fusi裡行on between the N-terminus o個商f the RET gene and the C-terminus o筆睡f the GRB2 gene, which 醫能resulted in production of a RET::G要熱RB2 fusion protein expres筆文sed in the tumor cells. Inter月裡estingly, GRB2 is known t西說o interact with and regula拿如te the function of筆志 RET in normal cells. &n拍什bsp;

The authors of the study the通的n further investigated 花著the function of 內哥the newly discovered fusion protein下拿 in vitro using mul呢輛tiple assays. Overall, they found tha影制t RET::GRB2 fusio子地n protein was activated in the absence 是懂of growth facto聽吧rs, promoted transformation of 做聽cells, and sensitized cells to RET inhi計雨bitors currently in u可一se in the clinic. This study近嗎 provides insight into how a子又 novel protein transforms cell如雨s and explores possible treatments for 睡窗pheochromocytoma.

Construction of 從樹the vectors used to clas視可sify the novel oncogenic prot吃資ein was enabled by Vec器章torBuilder’s Vector Design Studio. VectorBuilder offers cust東嗎om and pre-made lentiviral vectors and lentiviral pac店去kaging. Contact us to find out more or use our 坐個award-winning online pl車還atform to design草照 custom vectors s唱市pecific to your research needs算喝.

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Source (https://pubmed.ncbi.nlm.ni是有h.gov/35858593/)

Estrada-Zuniga CM, Cheng ZM, E音綠thiraj P, Guo Q, Gonzalez-Can亮請tú H, Add訊見erley E, Lopez H, Landry B對放N, Zainal A, Aronin N, 多務Ding Y, Wang X, Aguiar RCT, Dahia PLM. 玩物A RET::GRB2 fusion in pheo坐司chromocytoma defies the clas厭物sic paradigm of RET 街務oncogenic fusions. Cell Rep Med. 20現低22 Jul 19;3(7):100森遠686. doi: 10.1016/j.xcrm煙線.2022.100686. PMID: 3585校木8593.