New advances in molecular barcoding熱照 offer exciting公年 possibilities, especially動大 in cancer research. Ove業商r the years, molecular barcod民機ing involved generating spec服請ific nucleic acids or日月 proteins within cells t呢和o trace lineages across generations to 外事observe results. 訊外However, this approa兒林ch has required researchers to “kill”兵鐵 cells to harvest signature有小 genetic information. Recen能些t advances make紅冷 it possible to observe how isolated 麗老cells—especially tumor 子些cells—can be observed l樹紅ive and in real-ti票城me.

How can this nov明友el approach be used? Ann機就a ​​Obenauf at the算短 Memorial Sloan Kettering Cancer綠如 Center was able會西 to generate a barcoded oncolytic城刀 cell line in mice, int還煙roduce a treatmen畫光t, and analyze the impact. Obenau業月f and her team were able to evaluate 那兒the effect treatment had and more門是 clearly understand長會 the mechanics behind mut在拿ations that resulted in 理玩drug resistance.懂知 Using CRISPR–Cas9 gene editing to tar北那get barcoded cell來歌s, Obenauf and her t又謝eam were then able 場服to draw an important co就路nclusion: tumor cells became問放 resistant to t草子reatment in resp店劇onse to the introduced drug,水東 providing direction for場謝 future follow-up treatment.

Similarly, Barb懂花ara Grüner and her team at Uni雪老versity Hospital Essen in Germany u到志sed barcoding to study the 區雪efficacy of tre用到atments after me票唱tastasization. Starting with pancrea司拍tic cancer, they到鐘 studied how a treatment reduced the友店 number of barcoded cells tha外窗t spread to lungs in mice. Thanks t要愛o the barcoding appro村唱ach, they were able to achieve風上 significant results working with a fr票從action of the number of mice that would票藍 have been required using other techn讀們iques.

Another approach has 去拍been to use barco電頻des to “illuminate” the spatial organiz草機ation of cancer. Garry Nolan and Julien著黑 Sage at Stanford Uni看用versity used protein-based barc土雪odes that relied on a combination校場 of antibodies associated靜亮 with a heavy-metal atom. The team us低妹ed multiplexed i拍錢on beam imaging (MIBI) to visualize thr玩是ee-peptide barcodes, and they were the自民n able to explore the st紙醫ructure of tumors.

VectorBuilder provides barcoded cell l吧城ines as well as CRISPR genome editi數習ng solutions. If yo討懂u’re ready to save time and money 睡紅while you focus o說機n your next life-saving, world-chan公道ging breakthrough, d暗答esign and order vectors for your next民文 experiment at VectorBuilder.com. 歌上

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Source: Madhusoodanan. Nature;快鐵 https://doi.org/10.1中答038/d41586-022-00798-不拿w (2022)